School Improvement in Maryland

ENGAGEMENT (1.a)
“Enzyme Specificity: Introduction to Enzymes”
 
Rationale:
  • To illustrate the specificity of enzyme action
     
Time:
  • Approximately 30 minutes
     
Objectives:
  • To introduce students to the concept of enzyme and substrate reactions
     
Materials: (for class of 30)
  • Assorted locks/keys (15)
  • Food Cards
  • Card stock and envelopes
  • Yarn
  • Students will use locks and matching keys to represent enzymes and substrates in human digestion.
     
Procedure:
  1. Fifteen students (or half of the class) are chosen to represent food items.
  2. Each student is given a sealed envelope with the name of the food item on it to wear.
  3. Students then line up according to food item type (which is indicated on the outside of the envelope).
  4. Each of these students is also given a specific lock.
  5. Fifteen other students (or remaining class members) are given keys.
  6. Students mingle attempting to match locks and keys.
  7. When a key opens a lock, the pair of students returns to their original position in line.
  8. After all locks and keys are matched, each person representing a food person opens his/her card and shares the enzyme/substrate information on the card with the class.

Food Enzyme
1. Coke sucrose/sucrase
2. French fries polysaccharide/amylase & lipid/lipase
3. Hamburger
  • bun
  • pickles
  • cheese
  • beef patty
  • ketchup
  • mustard
  •  
    polysaccharide/amylase & nucleic acid/nuclease
    cellulose/cellulase
    lipid/lipase and protein/protease & lactose/lactase
    protein/protease & lipid/lipase
    sucrose/sucrase
    sucrose/sucrase
    4. Ice cream maltose/maltase, lipid/lipase, and lactose/lactase

    Enzyme Food Matches
    1. Carbohydrates:
  • Sucrose
  • Maltose
  • Lactose
  • Polysaccharides
  • Cellulose
  •  
    sucrase
    maltase
    lactase
    amylase
    cellulase
    2. Proteins protease
    3. Lipids lipase
    4. Nucleic Acids nuclease

    Category of Food Matching Food No. of Locks
    A. Carbohydrates    
      1. Sucrose
     Coke
    Ketchup/Mustard
     2 identical
      2. Maltose
     Ice Cream 1
      3. Lactose
     Ice Cream
    Cheese
     2 identical
      4. Polysaccharides
     Fries
    Bun
     2 identical
      5. Cellulose
     Pickle 1
    B. Proteins Beef Patty
    Cheese
     2 identical
    C. Lipids Beef Patty
    Cheese
    Ice Cream
    Fries
     4 identical
    D. Nucleic Acids Bun 1 lock
     
    Information for Food Cards

    SODA (on exterior of envelope)
    (Inside envelope) Disaccharides are carbohydrates essential in supplying the body with energy. The energy contained in a disaccharide is released only when it is broken down by an enzyme. This enzymatic reaction takes place in the stomach and small intestine.

    A glass of soda can contain up to 50 grams of sucrose. Sucrose is a type of disaccharide that is composed of glucose molecules that are chemically bonded to fructose molecules. The only key that unlocks sucrose is sucrase.

    KETCHUP/MUSTARD
    Although, ketchup and mustard contain tomatoes and mustard seeds, respectively, their primary ingredients are sugar and water! The sugar that is most commonly found in both ketchup and mustard is sucrose. The only key that unlocks sucrose is sucrase.

    ICE CREAM (1)
    Ice cream is a colloid, a suspension of sugar molecules in frozen fat. Maltose is one type of sugar that may be found in ice cream. Maltose is a second type of disaccharide. Maltose molecules are formed from two glucose molecules that are chemically bonded. The only key that unlocks maltose is maltase.

    CHEESE (1)
    A type of sugar that is always found in cheese and other dairy products is lactose. Lactose is a third type of disaccharide. Lactose, is composed of glucose molecules that are chemically bonded to galactose molecules. Some people lack the enzyme required to break down lactose. We say that those people are “lactose intolerant.” The only key that unlocks lactose is lactase.

    ICE CREAM (2)
    Since ice cream is a dairy product, it also contains lactose. Lactose that is not broken down by the body's natural lactose is fermented by bacteria in the intestine. This process can produce large quantities of gas. The only key that unlocks lactose is lactase.

    FRENCH FRIES (1)
    French fries are made from potatoes. Potatoes are filled with starch. Plants produce starch as a way to store extra energy. Starch is a polysaccharide containing long chains of repeating units of glucose. One enzyme that unlocks starch is amylase.

    BUN (1)
    Buns contain starch and other complex carbohydrates. Starches are broken down by amylase. Amylase is found in saliva, so digestion starts the moment you begin to chew. Amylase is also produced by the pancreas. One enzyme that unlocks starch is amylase.

    PICKLE
    Pickles and all other vegetables contain the polysaccharide, cellulose. Cellulose is important in the structure of the cell wall of plants. Although cattle and other herbivores have the ability to digest cellulose, human beings lack the enzyme needed to break down cellulose. Therefore, cellulose passes undigested from the body human body. Cellulose provides the fiber or roughage that is an important component of the human diet. The only key that unlocks cellulose is cellulase.

    BEEF PATTY (1)
    Meat is the muscle tissue of an animal. Meat is composed primarily of proteins, which are, in turn, composed of amino acids. Like the letters of the alphabet, which are combined in nearly infinite ways to form words, amino acids are combined to form proteins. There are thousands of different proteins yet only 20 different amino acids. The enzymes that unlock proteins are collectively called proteases. The only key that unlocks a protein is a protease.

    CHEESE (2)
    The protein that is found in cheese was assembled by a cow from the amino acids she consumed in her diet. After digesting protein, humans also reassemble the component amino acids into human proteins; thus, the expression, “You are what you eat.” The only key that unlocks a protein is a protease.

    BEEF PATTY (2)
    As much as 30% of a beef patty is fat. This fat was stored by a cow for its future energy needs. The excess fat that we eat goes directly to OUR storage areas. The group of enzymes that break down lipids are called lipases. The only key that unlocks a lipid (fat) is a lipase.

    CHEESE (3)
    Cheese is made from the fat that is skimmed from the surface of raw milk (milk that has not been homogenized). In fact, the main ingredient in cheese is milk fat. Fat is a lipid. The only key that unlocks a lipid is a lipase.

    ICE CREAM (3)
    When raw milk settles, cream floats to the top. This cream is used to make ice cream. When milk is used instead, the product is called “ice milk.” Ice milk contains less fat than ice cream. Cream is a lipid. The only key that unlocks a lipid is a lipase.

    FRENCH FRIES (2)
    Although potatoes contain no fat, french fries are among the fattiest of foods that you can eat. The fat is added when the potato strips are deep-fried. The only key that unlocks a lipid is a lipase.

    BUN (2)
    Nucleic acids include DNA and RNA, the molecules that provide the “blueprints” for all organisms. Because they are found in all living things, nucleic acids are present in all foods. The enzymes that break down nucleic acids are called nucleases. The only key that unlocks a nucleic acid is a nuclease.


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