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“Factors That Affect the Activity of Enzymes”
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| A student conducted an experiment to measure the reaction rate of the enzyme catalase on a solution of hydrogen peroxide. In this reaction, the enzyme facilitates the breakdown of hydrogen peroxide into water and oxygen. The reaction is illustrated by the following chemical equation:
The release of oxygen causes a change in pressure which can be measured by a CBL apparatus. Data from this reaction are shown in the table below.
THE CHANGE IN PRESSURE CAUSED BY THE REACTION
OF CATALASE AND HYDROGEN PEROXIDE
|
Time
(seconds) | Pressure
(mm Hg) |
| 0 | 0 |
| 2 | 8 |
| 4 | 16 |
| 6 | 24 |
| 8 | 32 |
| 10 | 40 |
| 12 | 48 |
| 14 | 48 |
| 16 | 48 |
| 18 | 48 |
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- Use the data provided to construct a line graph of this reaction. Be sure to include all of the elements specified on the "Scoring Criteria for Graphs" sheet.
- Next, select one of the following variables and describe how it would affect the rate of the reaction.
Variables:
- The temperature drops
- The pH drops
- The substrate concentration increases
- Finally, draw a dashed line on the graph to illustrate the change that would occur.
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