School Improvement in Maryland


EXPLORATION (2.b)
“Teaching Enzyme Action Using CBL Technology”
 
Rationale:
  • To explore the reaction rate of the enzyme, catalase, on the breakdown of hydrogen peroxide
     
Time:
  • Approximately 30-40 minutes
     
Objectives:
  • To measure the action of an enzyme (catalase) on its substrate (hydrogen peroxide)
  • To prepare a catalase solution from fresh liver
  • To collect data using a CBL.
     
Materials: (for class of 30)
  • CBL/TI system
  • CBL/TI system
  • Vernier Gas Pressure Sensor
  • fresh liver
  • hydrogen peroxide
  • see lab for additional materials
     
Overview:
  • Students will monitor the reaction by recording pressure changes as oxygen gas is given off during the reaction.
     
Procedure:
    See Thornton, M. 1998. “Teaching Enzyme Action Using CBL Technology.”
    (See Attachment for Teacher Preparation)
    (See Attachment for Student Lab)

return.