Rationale:

• To explore the reaction rate of the enzyme, catalase, on the breakdown of hydrogen peroxide
   

Time:

• Approximately 30-40 minutes
   

Objectives:

• To measure the action of an enzyme (catalase) on its substrate (hydrogen peroxide)
• To prepare a catalase solution from fresh liver
• To collect data using a CBL.
   

Materials: (for class of 30)

• CBL/TI system
• CBL/TI system
• Vernier Gas Pressure Sensor
• fresh liver
• hydrogen peroxide
• see lab for additional materials
   

Overview:

• Students will monitor the reaction by recording pressure changes as oxygen gas is given off during the reaction.
   

Procedure:

See Thornton, M. 1998. “Teaching Enzyme Action Using CBL Technology.”
(See Attachment for Teacher Preparation)
(See Attachment for Student Lab)