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“Proof of Enzyme Action”
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| Rationale: |
- To explore the effect of an enzyme on its substrate
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| Time: |
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| Objectives: |
- To use models to demonstrate the effect of an enzyme on its substrate (starch molecules will be broken down into their component monosaccharide units).
- To use Benedict's solution to test for the presence of starch and glucose
- To look for evidence of enzyme action by testing an starch/amylase solution
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| Materials: |
- starch solution
- Benedict's solution
- iodine solution
- amylase
- hot plate
- see lab for additional materials
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| Overview: |
- Students will use models to illustrate enzyme action.
- Students will test a starch solution with iodine and Benedict's solution
- Students will test a starch/amylase solution with iodine and Benedict's solution
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| Procedure: |
See Kaskel, A, Hummer, Jr. P., et al. 1994. Investigating Living Systems-
Laboratory Manual. “Proof of Enzyme Action.” Glencoe. 35-38.
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