Rationale:

• To explore the effect of an enzyme on its substrate
   

Time:

• Approximately 45 minutes
   

Objectives:

• To use models to demonstrate the effect of an enzyme on its substrate (starch molecules will be broken down into their component monosaccharide units).
• To use Benedict's solution to test for the presence of starch and glucose
• To look for evidence of enzyme action by testing an starch/amylase solution
   

Materials:

• starch solution
• Benedict's solution
• iodine solution
• amylase
• hot plate
• see lab for additional materials
   

Overview:

• Students will use models to illustrate enzyme action.
• Students will test a starch solution with iodine and Benedict's solution
• Students will test a starch/amylase solution with iodine and Benedict's solution
   

Procedure:

See Kaskel, A, Hummer, Jr. P., et al. 1994. Investigating Living Systems- Laboratory Manual. “Proof of Enzyme Action.” Glencoe. 35-38.